By Z. Mojok. City University of Los Angeles. 2018.
This method was advantageous in that a single fluorescein-labelled conjugate with a long shelf-life could be used to measure antibodies to any disease in a particular species 100 mcg cytotec otc symptoms 5dpo. The test could be carried out quickly on small test samples and the visual reading permitted recognition of particular histological staining patterns (e purchase 100mcg cytotec amex treatment tendonitis. However, immunofluorescence suffers from the fact that it is very labour intensive; the reading is subjective; and it is essential to use a fluorescence microscope. Some of these difficulties have been largely overcome in that microscopical fluorescence-intensity reading equipment has been developed and low-cost fluorescence microscopes with less demanding illumination systems are now available. A further development has been the introduction of fluorimeters which can be used to measure fluorescence on solid-phase immobilized antigens. In one popular system the soluble antigen is attached to a plastic “paddle” device  which is used with a dedicated fluorimeter. With such devices accurate objective estimation of immunofluorescence can be achieved. The other major development in immunofluorescence is the introduction of monoclonal antibodies for use in direct or sandwich assays. To date these have been used both in fluorescence cell sorters and in normal microscopical immunofluorescence tests. Fluorescein-labelled monoclonal antibodies (or unlabelled monoclonals in fluorescence sandwich methods) are now being generated against a range of microbial organisms . We can anticipate their rapid introduction for specific identification of pathogens and for measuring antibody responses. Fluoroimmunoassay  methods for the detection of analytes in solution have been the subject of rapid development over the last five years. Attention has been focused on both homogeneous assays (without separation steps) and heterogeneous assays (with separation steps). In the homogeneous systems fluorescence is increased or, more commonly, decreased when the labelled material participates in a specific immunological reaction. Indirect fluorescent quenching assays are also being developed for large protein molecules including antibodies . Other novel homogeneous fluoroimmunoassays including fluorescence excitation transfer immunoassay  which again can be used to assay proteins. The heterogeneous fluoroimmunoassays, which involve separation steps, have been used to measure drugs (e. One of the most promising developments is the use of those labels which emit fluorescence for a relatively long time after excitation (e. With the appropriate time-resolution equipment it is then easy to distinguish the “specific signal” fluorescence from other “non-specific” background fluorescence . Luminescent labels should also be considered in the same context as fluorophors since many of the merits and disadvantages are similar. Luminescence can also be detected easily at very low levels and now many luminometers are becoming available. Luminescence, release of light from a label, can either be generated chemically —chemiluminescence ; or biologically —bioluminescence. To date numerous research applications of luminescence immunoassay have been published  but they have not come into general use.
Most men at some point in their sexual development (usually their teen years) experience nocturnal emissions (wet dreams at night) buy cheap cytotec 100 mcg online medicine ball core exercises. Although psychological factors obviously contribute to the male sexual response purchase cytotec 100 mcg otc treatment goals for ptsd, it is interesting to note that they are not absolutely necessary in the performance of the male sexual act. Appropriate genital stimulation can lead to an erection and ejaculation without psychic stimuli through an inherent reﬂex mechanism. For example, some individuals with spinal cord damage that prevents the transmission of nerve impulses from the brain are still capable of achieving an erection and ejaculation. Physical stimulation of sensitive tissue, primarily the penis but also the entire pubic region, sends nerve impulses to the spinal cord, causing a reﬂex impulse to the penis that leads to dilation of the arteries and the ﬁlling up with blood of the erectile tissue. In addition, these same nerve impulses cause the glands in the urethra to secrete mucus that lubricates the urethra and also aids in the lubrication of intercourse. The initial nerve stimulus from the spinal cord during the sexual act is controlled by the parasympathetic nervous system, which also controls bodily functions such as digestion, breathing, and heart rate during periods of rest, relaxation, visualization, meditation, and sleep. In contrast, the sympathetic nervous system is designed to protect us against immediate danger and is responsible for the so-called ﬁght-or-ﬂight reaction. While the parasympathetic nervous system is responsible for an erection and lubrication, the sympathetic nervous system controls emission and ejaculation. When sexual stimulation becomes extremely intense, the reﬂex centers of the spinal cord begin to emit sympathetic nerve impulses to initiate emission, the forerunner of ejaculation. Emission begins with contraction of the vas deferens, the tubule that transports the sperm from the epididymis to the prostate. This contraction leads to the expulsion of sperm into the ejaculatory duct and urethra. Then contractions of the prostate and seminal vesicles expel prostatic and seminal ﬂuid into the ejaculatory duct, forcing the sperm into the urethra. All of these ﬂuids mix in the internal urethra along with the secretions of the urethral glands to form semen. The ﬁlling of the urethra then elicits sensory nerve impulses that further excite the rhythmic contractions of the internal organs and also cause the rhythmic contraction of the erectile tissues. Together, these contractions lead to a tremendous increase in pressure that ejaculates the semen from the urethra. Simultaneously, the pelvic muscles and even muscles of the abdomen cause thrusting movements of the pelvis and penis, which also help propel the semen. After ejaculation, the male sexual excitement disappears almost entirely within one or two minutes, and erection disappears. In the overwhelming majority of cases the cause is organic, that is, it is due to some physiological dysfunction. In fact, in men over the age of 50, organic causes are responsible for erectile dysfunction in more than 90% of cases. Causes of Erectile Dysfunction Organic (90%) • Vascular insufficiency Atherosclerosis Pelvic surgery Pelvic trauma • Drugs Antihistamines Antihypertensives Anticholinergics Antidepressants Antipsychotics Tranquilizers Others • Alcohol and tobacco use • Endocrine disorders Diabetes Hypothyroidism Decreased male sex hormones Elevated prolactin levels High serum estrogen levels • Diseases of or trauma to the sexual organs Diseases of the penis Prostate disorders • Neurological diseases • Pelvic trauma • Pelvic surgery • Multiple sclerosis Psychological (10%) • Psychiatric illness • Stress • Performance anxiety • Depression Since correction of any underlying organic factor is the ﬁrst step in restoring sexual function, it is critically important that a proper diagnosis be made. A thorough history and physical exam are most often all that is needed; however, there are special noninvasive tests that can be performed to diagnose the cause of erectile dysfunction. Procedures Used to Evaluate Erectile Dysfunction • Medical history • Physical examination • Laboratory studies Complete blood count and urinalysis Biochemical profile Glucose tolerance test Serum hormone levels • Psychological evaluation • Nighttime penile monitoring • Neurological examination • Vascular examination Atherosclerosis of the penile artery is the primary cause of impotence in nearly half the men over the age of 50 who have erectile dysfunction. Atherosclerosis-related erectile dysfunction has been shown to be a risk factor for a heart attack or stroke. Patients with diseased coronary arteries are much more likely to have erectile dysfunction than individuals without coronary disease. If erectile dysfunction is due to vascular insufﬁciency, especially important are measures to reduce cardiovascular risk factors such as elevated cholesterol and triglyceride levels, high blood pressure, obesity, lack of exercise, and smoking. The diagnosis of erectile dysfunction due to atherosclerosis can be made with the aid of ultrasound techniques.
Hatchability Immaturity purchase cytotec 200mcg without prescription treatment bronchitis, pair incompatibility 100mcg cytotec with amex symptoms of anxiety, normal species differences, normal rates can be calculated for individual pairs, separate occurrence as part of clutch, sexual inexperience, lack of early clutches, different species, eggs incubated naturally, learning, aviary disturbances, lack of social interaction, excess eggs incubated artificially and eggs that had various social interaction, homosexual pairs, lack of pair-bonding, asynchro- nous breeding condition, improper imprinting, infrequent matings. The more precise the Environmental: hatchability statistic, the more diagnostic the infor- Incorrect photoperiod, incorrect nest box design or nesting mate- rials, incorrect enclosure design, lack of visual barriers, excessive mation that is provided (Figure 29. In domestic fowl, the hatchability of naturally and artificially incubated fertile eggs approaches 85 to Medical: 90%. With companion and aviary birds, this figure Obesity, age (young or old breeders), inbreeding, vent feathers, drug therapy (causing vitamin deficiency or direct, decreased may be much lower, and ranges from 8% to 100% fertility), previous hormonal therapy (testosterone injections), have been discussed. The number of lethal or chromosomal abnormalities reported in companion bird species is low when com- The fertility rates of most free-ranging companion pared to domestic species. Evaluating fertility and birds have not been determined, although in some hatchability statistics from parents and sisters of species studies have indicated that fertility can be breeding males may help identify lethal or semi-le- quite high. Breeding tests may the potential for similar fertility rates but more commonly the rates are lower, probably due to a combina- tion of environmental and dietary factors. This cyclic produc- tion is probably related to environ- mental factors and not due to disease-related infertility. Avicultur- ists should establish their own fertil- ity rates and standardize data so that comparisons can be made among similar aviaries. Hatchability Hatchability rates are determined from eggs that were known to be fer- tile. The egg on the left was from a are calculated by finding the percent- normal unassisted hatch, and the chick from this egg was strong and developed normally post-hatching. Exposure to toxic compounds, either directly – Cooling after development has begun or in the food or water, should be considered. Behav- – Suffocation due to incorrect ventilation Inbreeding ioral problems including lack of pair-bonding, incon- Chromosome abnormalities sistent parental incubation and egg trauma in the Egg-transmitted infectious diseases nest may also cause hatchability problems. Total caloric intake and food selection – Riboflavin, vitamin B12, folic acid, biotin, manganese, behavior for each individual bird should be evalu- pyridoxine, pantothenic acid, phosphorous, boron, li- ated. Nutritionally deficient hens can produce eggs, noleic acid, vitamin K, vitamin D Secondary vitamin deficiencies but the low level of nutrients may prevent the eggs – Antibiotic therapy destroying vitamin-producing flora from hatching. The age of embryonic mortality will – Diet imbalances, inadequate food intake usually depend on the degree and type of deficiency Viral diseases Bacterial infections or toxicity. Fungal infections Egg jarring or shaking in the first trimester Severe hypovitaminosis A causes a complete cessa- Incubator faults tion of egg production. Vita- Malpositions min E deficiencies can cause lethal rings in which the – Inadequate or incorrect turning embryo is seen surrounded by a ring of separated – Abnormal egg size or shape – Incorrect incubator temperature tissue. Vitamin D3 deficiencies can cause small eggs Incubator faults with poorly calcified shells. Ultraviolet light expo- – Poor incubator ventilation sure may improve hatchability in these cases while – Egg cooling early in incubation – Inadequate or incorrect turning excess D3 may lead to a complete cessation of egg – Incorrect temperature production. Embryonic hemorrhage is common with – Incorrect humidity deficiencies in vitamins E and K. Vitamin K is also Incorrect hatcher temperature or humidity Long storage time pre-incubation involved with calcium transport, and vitamin K defi- Infectious disease ciencies can mimic the clinical signs associated with Nutritional deficiencies hypocalcemia. The calcium/manganese ratio regulates the rate of be required to establish whether such genes are sex- hatching, and imbalances of these minerals may linked or autosomal, dominant or recessive. Given the wide variabil- ity in the types of food (and thus the composition of Parental Factors these foods) consumed by free-ranging birds of differ- The medical history of each parent should be exam- ent species, it is not surprising that a single commer- ined to identify factors that may affect fertility and cially available diet cannot meet the needs of all hatchability. It is specu- eggs that are artificially incubated for the entire lated that breeding third and fourth generations of developmental period (Figure 29. The fact that companion bird species will result in higher fertility different hatchability rates exist between natural and hatchability rates in birds fed commonly avail- and artificially incubated eggs highlights the need able commercial diets (see Chapters 3 and 31).
Another observation is the irregular necrosis of the reticular cells in the lympho- cytically depleted spleen buy cytotec 200mcg without a prescription medications drugs prescription drugs, which would suggest per- manent immunosuppression generic cytotec 200mcg with amex medications 563. Experimentally infected Rose-breasted Cockatoo neonates became acutely depressed and anorectic approximately four weeks post-infection. Twenty- four hours later, the feathers appeared to lose their luster and became pale and brittle. Subsequently, dystrophic feathers began to appear as the neonates developed their adult plumage. These birds may appear totally normal Birds with long-term infections frequently appear bald as feather one day and exhibit 80 to 100% feather dystrophy pathology progresses through successive molts. Gross changes include reten- necropsy and thorough histologic exam are not per- tion of feather sheaths, hemorrhage within the pulp formed on young of susceptible species that die sud- cavity, fractures of the proximal rachis and failure of denly. Free-ranging birds with severe feather Acute infections are characterized by several days of pathology may have an accompanying brownish dis- depression followed by sudden changes in developing coloration of the skin that is thought to occur from feathers, including necrosis, fractures, bending, exposure of normally sheltered skin to sunlight. This clinical picture feathers with dystrophic, necrotic, non-viable feath- is particularly common in young Sulphur-crested ers that stop growing shortly after emerging from the Cockatoos and lovebirds. In contrast to the classic presen- tation just described, some birds have substantial involvement of the flight, tail and crest feathers, with only minimal changes in the powder down feath- ers. Necrosis of the upper beak progresses fractures of the proximal rachis and failure of the developing proximally to the palatine area and may involve the feather to exsheath; however, it must be stressed that any damage premaxilla in severe cases. The distal end of the to the follicular epithelium can cause a similar appearing gross 187 lesion. Polyomaviral, adenoviral, bacterial down feathers in cockatoos are dystrophic, the beak and fungal folliculitis can cause similar lesions. The beak may elongate or show the feather shaft, where necrosis and ballooning de- transverse delamination or fractures, with or with- generation of epithelial cells in the epidermal collar out bacterial or fungal infections in the clefts (see and epidermal, basal and intermediate zones of the Figure 19. The follicu- Likewise, deformities, fractures, necrosis and 230 lar epithelium may also be necrotic, but this lesion is sloughing of the nails can be seen occasionally. In one study involving 22 cocka- to severe bursal or thymic necrosis with the presence toos of mixed Asian origin, birds older than one year of viral-induced inclusion bodies. Feather pathology of age had a lower incidence of beak lesions than did in these cases may not occur, or may be limited to birds that were under one year of age. In of viral antigen within macrophages in the bone mar- young birds, the cloacal bursa may be small with row and within circulating monocytes suggests that poorly developed folds and the thymus may reveal these cells may be directly infected (Color 32. In mature birds the spleen is frequently small and depleted of lym- Diagnosis phocytes, and occasionally necrosis of the reticular Feather lesions that appear grossly similar can be cells can be observed. In the intestinal tract inclusion bodies were fectious causes of similarly appearing feather lesions mainly found in epithelial cells. Precipitat- viral-specific antibody staining in the beak, hard ing antibodies can be demonstrated using an agar-gel palate, bursa, thymus, tongue, parathyroid gland, immunodiffusion test (see Figure 32. Recoveries have been and has no feather abnormalities must be retested in reported principally in birds with only intranuclear 90 days. A negative test 90 ated as long as the animal is kept in a controlled days later would indicate that the viral nucleic acid environment, beak lesions (also nail lesions) can be was no longer detected in the blood and that the bird painful, particularly when secondarily infected. These birds should be restricted from contact with other Control susceptible birds, particularly neonates. Infected birds should be removed and infectivity remains unchanged when the virus is from the breeding collection and nursery immedi- heated to 60°C for one hour and following treatment ately (see Figure 30. This maternally derived antibodies to their chicks that is particularly true with respect to breeding birds, offer at least temporary immunity to the virus (Color birds being sent to pet shops and birds being evalu- 32.
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